Microfluidic single-cell real-time PCR for comparative analysis of gene expression patterns

  title={Microfluidic single-cell real-time PCR for comparative analysis of gene expression patterns},
  author={Veronica Sanchez-Freire and Antje D. Ebert and Tomer Kalisky and Stephen R Quake and Joseph C Wu},
  journal={Nature Protocols},
Single-cell quantitative real-time PCR (qRT-PCR) combined with high-throughput arrays allows the analysis of gene expression profiles at a molecular level in approximately 11 h after cell sample collection. We present here a high-content microfluidic real-time platform as a powerful tool for comparatively investigating the regulation of developmental processes in single cells. This approach overcomes the limitations involving heterogeneous cell populations and sample amounts, and may shed light… CONTINUE READING
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