Microfluidic extraction, stretching and analysis of human chromosomal DNA from single cells.

@article{Bentez2012MicrofluidicES,
  title={Microfluidic extraction, stretching and analysis of human chromosomal DNA from single cells.},
  author={Jaime J Ben{\'i}tez and Juraj Topolancik and Harvey C Tian and Christopher B Wallin and David R. Latulippe and Kylan Szeto and Patrick J M Murphy and Benjamin R. Cipriany and Stephen L. Levy and Paul D. Soloway and Harold G. Craighead},
  journal={Lab on a chip},
  year={2012},
  volume={12 22},
  pages={4848-54}
}
We describe a microfluidic device for the extraction, purification and stretching of human chromosomal DNA from single cells. A two-dimensional array of micropillars in a microfluidic polydimethylsiloxane channel was designed to capture a single human cell. Megabase-long DNA strands released from the cell upon lysis are trapped in the micropillar array and stretched under optimal hydrodynamic flow conditions. Intact chromosomal DNA is entangled in the array, while other cellular components are… CONTINUE READING

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