Microfluidic diagnostic technologies for global public health

  title={Microfluidic diagnostic technologies for global public health},
  author={Paul Yager and Thayne L. Edwards and Elain Fu and Kristen L Helton and Kjell E. Nelson and Milton R. Tam and Bernhard H. Weigl},
The developing world does not have access to many of the best medical diagnostic technologies; they were designed for air-conditioned laboratories, refrigerated storage of chemicals, a constant supply of calibrators and reagents, stable electrical power, highly trained personnel and rapid transportation of samples. Microfluidic systems allow miniaturization and integration of complex functions, which could move sophisticated diagnostic tools out of the developed-world laboratory. These systems… 
Microfluidic diagnostics for the developing world.
Some of the latest breakthroughs that could significantly impact medical diagnostics in the developing world are discussed.
Modular microfluidic cartridge-based universal diagnostic system for global health applications
An open platform diagnostic system which allows to run molecular, immunological and clinical assays on a single instrument platform with a standardized microfluidic cartridge architecture in an automated sample-in answer-out fashion is developed.
Microfluidics-based diagnostics of infectious diseases in the developing world
The 'mChip' assay had excellent performance in the diagnosis of HIV using only 1 μl of unprocessed whole blood and an ability to simultaneously diagnose HIV and syphilis with sensitivities and specificities that rival those of reference benchtop assays.
Microfluidics at the crossroad with point-of-care diagnostics.
Two sets of technologies are described which comply with the constraints of the POC environment and illustrate a possible route for the development of microfluidic devices, which could fulfil the needs of clinicians for disease staging and monitoring.
Microfluidic Diagnostic Systems for the Rapid Detection and Quantification of Pathogens
This article reviews past and current research directed towards developing microfluidic systems that are able to rapidly detect the presence of pathogens and provide additional clinically relevant
Microfluidic diagnostics for low-resource settings
An overview of existing platform development efforts is given, some original research in this area at PATH is presented, and a call to action for more is reiterated.
In vitro diagnostic platforms of the future; technological possibilities and challenges
  • L. Furuberg, S. M. Borch
  • Biology
    Proceedings of the 6th International Workshop on Wearable, Micro, and Nano Technologies for Personalized Health
  • 2009
This work presents examples of platforms analyzing patient samples for proteins and nucleic acids, and some of the challenges involved in volume manufacturing of these platforms.
Point-of-Care Diagnostics in Low Resource Settings: Present Status and Future Role of Microfluidics
This review will critically survey and analyse the current lateral flow-based point-of-care (POC) technologies, which have made a major impact on diagnostic testing in developing countries over the last 50 years and the future of POC technologies including the applications of microfluidics.


Some of the emerging principles for manipulating blood cells at microscale and promising high-throughput approaches to blood cell separation using microdevices are reviewed.
Protein biochip systems for the clinical laboratory
Current trends and advances in miniaturized multiplexed immunoassay technologies are presented, reviewing different systems from research to point-of-care assays and focusing on a subset of chip-based assays that may be used in a clinical laboratory and are directly applicable for biomedical diagnosis.
Applying Microfluidic Chemical Analytical Systems to Imperfect Samples
Over the last 4 years our group has been involved in developing a series of devices for chemical separation and analysis. These devices share a theme of utilizing the low Reynolds number properties
Fully integrated multiplexed lab-on-a-card assay for enteric pathogens
Progress to date on sample and bacteria processing methodologies, identification and validation of capture antibodies and strategy for organism immunocapture, and implementation of on-chip nucleic acid extraction for a subset of enteric pathogens are reported on.
Point-of-care immunotesting: approaching the analytical performance of central laboratory methods.
Significant advances in assay and detection technologies have recently facilitated the introduction of truly quantitative, sophisticated immunoassay methods to POC settings as well, with the analytical performance characteristics approaching those of conventional laboratory assays.
Controlled microfluidic reconstitution of functional protein from an anhydrous storage depot.
A novel method has been developed for preserving molecules in microfluidic devices that also enables the control of the spatial and temporal concentrations of the reconstituted molecules within the
Single-molecule fluorescence detection in microfluidic channels—the Holy Grail in μTAS?
Both single-molecule detection (SMD) methods and miniaturization technologies have developed very rapidly over the last ten years. By merging these two techniques, it may be possible to achieve the
A Planar Microfabricated Fluid Filter
Many blood tests must be performed on plasma without cellular matter present. In the standard laboratory protocol, pure plasma is obtained through centrifugation. In order to produce a miniaturized
A Microchip CD4 Counting Method for HIV Monitoring in Resource-Poor Settings
The development of a prototype for a simple, rapid, and affordable method for counting CD4 lymphocytes with close agreement with standard flow cytometry, and the ability to discriminate clinically relevant CD4 count thresholds with high sensitivity and specificity is described.