The fraction collection of DNA fragments for studying gene expression, suffered from technical difficulties, including those related to the accurate capture of fast moving targets and pure collection under conditions of insufficient separation. We introduces a new concept of simultaneous space sampling for capturing targets in complicated situation, like overlapping fragments. Ten parallel extraction channels which covered 1.5 mm long sampling ranges were used to facilitate the capturing of fast moving fragments. Furthermore, the space-sampling extraction made it possible to acquire pure collection, even from partly overlapping fragments that had been insufficiently separated after a short electrophoretic run. Three fragments differed in size by one base bases, were simultaneously collected within 10 min.