Microfilament disruption is required for enucleation and nuclear transfer in germinal vesicle but not metaphase II human oocytes.

@article{Tesak2003MicrofilamentDI,
  title={Microfilament disruption is required for enucleation and nuclear transfer in germinal vesicle but not metaphase II human oocytes.},
  author={Jan Tesař{\'i}k and Francisco Mart{\'i}nez and Laura Francesca Rienzi and Filippo Mari Ubaldi and Marcello Iacobelli and Carmen C. Jim{\'e}nez Mendoza and Ermanno Greco},
  journal={Fertility and sterility},
  year={2003},
  volume={79 Suppl 1},
  pages={
          677-81
        }
}
OBJECTIVE To evaluate the usefulness of microfilament disruption before enucleation and nuclear transfer in human oocytes at different stages of maturation. DESIGN Prospective experimental study. SETTING Private clinics. PATIENT(S) Infertile couples undergoing assisted reproduction attempts. INTERVENTION(S) Oocyte enucleation and nuclear transfer, activation of reconstructed oocytes. MAIN OUTCOME MEASURE(S) Oocyte survival, nuclear transfer efficacy, activation outcomes. RESULT(S… CONTINUE READING
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