Microfiberoptic fluorescence photobleaching reveals size-dependent macromolecule diffusion in extracellular space deep in brain.

@article{Zdor2008MicrofiberopticFP,
  title={Microfiberoptic fluorescence photobleaching reveals size-dependent macromolecule diffusion in extracellular space deep in brain.},
  author={Zsolt Z{\'a}dor and Mazin Magzoub and Songwan Jin and Geoffrey T. Manley and Marios C Papadopoulos and Alan S Verkman},
  journal={FASEB journal : official publication of the Federation of American Societies for Experimental Biology},
  year={2008},
  volume={22 3},
  pages={870-9}
}
Diffusion in brain extracellular space (ECS) is important for nonsynaptic intercellular communication, extracellular ionic buffering, and delivery of drugs and metabolites. We measured macromolecular diffusion in normally light-inaccessible regions of mouse brain by microfiberoptic epifluorescence photobleaching, in which a fiberoptic with a micron-size tip is introduced deep in brain tissue. In brain cortex, the diffusion of a noninteracting molecule [fluorescein isothiocyanate (FITC)-dextran… CONTINUE READING