Microfabricated device for DNA and RNA amplification by continuous-flow polymerase chain reaction and reverse transcription-polymerase chain reaction with cycle number selection.

@article{Obeid2003MicrofabricatedDF,
  title={Microfabricated device for DNA and RNA amplification by continuous-flow polymerase chain reaction and reverse transcription-polymerase chain reaction with cycle number selection.},
  author={Pierre J Obeid and Theodore K. Christopoulos and H. John Crabtree and Christopher J. Backhouse},
  journal={Analytical chemistry},
  year={2003},
  volume={75 2},
  pages={288-95}
}
We have developed a high-throughput microfabricated, reusable glass chip for the functional integration of reverse transcription (RT) and polymerase chain reaction (PCR) in a continuous-flow mode. The chip allows for selection of the number of amplification cycles. A single microchannel network was etched that defines four distinct zones, one for RT and three for PCR (denaturation, annealing, extension). The zone temperatures were controlled by placing the chip over four heating blocks. Samples… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 43 extracted citations

Development of a portable low-cost real-time PCR system

2017 International Conference on Robotics, Automation and Sciences (ICORAS) • 2017
View 1 Excerpt

Similar Papers

Loading similar papers…