Microenvironment-evoked cell lineage conversion: Shifting the focus from internal reprogramming to external forcing

  title={Microenvironment-evoked cell lineage conversion: Shifting the focus from internal reprogramming to external forcing},
  author={Ji Lin and Meirong Li and Dong-dong Ti and Mei-xia Chen and Hao-jie Hao and Ya-li Zhao and Xiao-bing Fu and Wei-dong Han},
  journal={Ageing Research Reviews},

Brief Azacytidine Step Allows The Conversion of Suspension Human Fibroblasts into Neural Progenitor-Like Cells

This work provides a simple way to induce neural progenitor-like cells from human fibroblasts without genetic manipulation by changing physicochemical culture properties from monolayer culture into a suspension in the presence of a chemical DNA methyltransferase inhibitor agent, Azacytidine.

Transdifferentiation of pancreatic cells by loss of contact-mediated signaling

This study provides a theoretical framework to understand the role of contact-mediated signaling in pancreatic cell fate control that may help to improve acinar-to-islet cell transdifferentiation strategies for β-cell neogenesis.

Synthesis of 6-O-poly(ϵ-caprolactone)-L-ascorbic acid and its controlled release from supramolecular polymer micelles.

A cytotoxicity study proves the excellent cytocompatibility of AA-6-PCL loaded β-CD/PCL SMPMs, which altogether herald their potential application in the study of the induced pluripotent stem cells.

Non-viral Transfection and Direct Reprogramming of Fibroblasts to Neurons and Glia: Importance of Physical and Chemical Microenvironments

An abstract of a dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the Department of Biomedical Engineering in the Graduate School of Duke University.



Turning straw into gold: directing cell fate for regenerative medicine

Two main approaches to accomplishing cell-replacement therapy are reviewed: in vitro directed differentiation, which is used to push pluripotent stem cells, including embryonic stem cells or induced pluripOTS, through steps similar to those that occur during embryonic development; and reprogramming, in which a differentiated cell is converted directly into the cell of interest without proceeding through a pluripotency intermediate.

Forcing cells to change lineages

The ability to produce stem cells by induced pluripotency (iPS reprogramming) has rekindled an interest in earlier studies showing that transcription factors can directly convert specialized cells

Reprogramming with defined factors: from induced pluripotency to induced transdifferentiation.

The available evidence for 'induced transdifferentiated cells' as a novel tool for adult cell fate modification is explored and the initial objections of iPS cells are summarized including their extremely low efficiency and the risk of tumor generation.

Progress toward the clinical application of patient-specific pluripotent stem cells.

The current state of iPS cell technology is described, including approaches by which they are generated and what is known about their biology, and the potential applications of these cells for disease modeling, drug discovery, and, eventually, cell replacement therapy are discussed.

Evolution of induced pluripotent stem cell technology

iPSC technology provides unprecedented opportunities in biomedical research and regenerative medicine, however, there remain a great deal to learn about iPSC safety, the reprogramming mechanisms, and better ways to direct a specific reprograming process.

A role for chemistry in stem cell biology

Cell-based phenotypic and pathway-specific screens of natural products and synthetic compounds have recently provided a number of small molecules that can be used to selectively control stem cell proliferation and differentiation, which may provide new insights into stem cell biology.

Stem cell states, fates, and the rules of attraction.