Microdissection of banded human chromosomes

  title={Microdissection of banded human chromosomes},
  author={Gabriele Senger and H. -J. L{\"u}decke and Bernhard Horsthemke and Uwe Claussen},
  journal={Human Genetics},
Physical dissection of metaphase chromosomes is the most straightforward approach for the isolation of DNA sequences from specific chromosome regions. However, conventional microdissection techniques are too crude and inefficient for analysis of the human genome. Here we describe a technique for the precise dissection of single bands from GTG-banded chromosomes. Cells from normal amniotic fluid cell cultures are harvested by the pipette method. Microdissection is performed on an inverted… CONTINUE READING


Publications citing this paper.
Showing 1-10 of 33 extracted citations


Publications referenced by this paper.
Showing 1-10 of 20 references

Construction and characterization of band-specific DNA libraries

  • H-J Liidecke, G Senger, U Claussen, B Horsthemke
  • Hum Genet
  • 1990

Microdissection of potential gene sequences from the Prader-Willi syndrome chromosome region

  • K Buiting, M Neumann, +5 authors B Horsthemke
  • Genomics
  • 1989
1 Excerpt

Mendelian inheritance in man, 8th edn

  • V McKusick
  • 1988
1 Excerpt

Microdis - section and microcloning of the long arm of human chromosome 7

  • R Kaiser, J Weber, +5 authors K Olek
  • Mol Biol Rep
  • 1987

Similar Papers

Loading similar papers…