Microdissection of banded human chromosomes

@article{Senger1990MicrodissectionOB,
  title={Microdissection of banded human chromosomes},
  author={Gabriele Senger and H. -J. L{\"u}decke and Bernhard Horsthemke and Uwe Claussen},
  journal={Human Genetics},
  year={1990},
  volume={84},
  pages={507-511}
}
Physical dissection of metaphase chromosomes is the most straightforward approach for the isolation of DNA sequences from specific chromosome regions. However, conventional microdissection techniques are too crude and inefficient for analysis of the human genome. Here we describe a technique for the precise dissection of single bands from GTG-banded chromosomes. Cells from normal amniotic fluid cell cultures are harvested by the pipette method. Microdissection is performed on an inverted… CONTINUE READING

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