Microdissection of Drosophila polytene chromosomes for DOP-PCR.

  title={Microdissection of Drosophila polytene chromosomes for DOP-PCR.},
  author={Abby F. Dernburg},
  journal={Cold Spring Harbor protocols},
  volume={2012 3},
This protocol describes a method for the microdissection of Drosophila polytene chromosomes. The microdissected DNA can be amplified by degenerate oligonucleotide primed-polymerase chain reaction (DOP-PCR) and then used to prepare probes for fluorescent in situ hybridization (FISH).