Microdialysis coupled on-line to capillary liquid chromatography with tandem mass spectrometry for monitoring acetylcholine in vivo.

@article{Shackman2007MicrodialysisCO,
  title={Microdialysis coupled on-line to capillary liquid chromatography with tandem mass spectrometry for monitoring acetylcholine in vivo.},
  author={Holly M Shackman and Minshan Shou and Nicholas A Cellar and Christopher J E Watson and Robert T Kennedy},
  journal={Journal of neuroscience methods},
  year={2007},
  volume={159 1},
  pages={86-92}
}
Capillary liquid chromatography-mass spectrometry (cLC-MS) was coupled on-line to microdialysis sampling to monitor endogenous acetylcholine (ACh) from the rodent brain. In vivo microdialysate sampled at 0.6 microL/min from the striatum of ketamine or chloral hydrate anesthetized rats was loaded onto a sample loop and then injected onto a approximately 5 cm long strong cation exchange (SCX) capillary column. A step gradient was used to separate the analyte from ionization suppressing salts… CONTINUE READING

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