Micrococcal nuclease as a probe of DNA sequence organization and chromatin structure.

@article{Keene1981MicrococcalNA,
  title={Micrococcal nuclease as a probe of DNA sequence organization and chromatin structure.},
  author={Michael A Keene and Sarah C. R. Elgin},
  journal={Cell},
  year={1981},
  volume={27 1 Pt 2},
  pages={57-64}
}
We have investigated micrococcal nuclease digestion of chromatin and purified DNA at the heat-shock locus 67B in Drosophila melanogaster. At early stages of the reaction a distinct set of fragments is generated, indicating the presence of preferential cleavage sites. These sites are also observed when purified recombinant plasmid DNA is used as the substrate, demonstrating that the sites are specified by the DNA sequence. At Drosophila locus 67B, prominent sites occur frequently, spaced… CONTINUE READING