Microchip device for cell lysis, multiplex PCR amplification, and electrophoretic sizing.

@article{Waters1998MicrochipDF,
  title={Microchip device for cell lysis, multiplex PCR amplification, and electrophoretic sizing.},
  author={Larry C Waters and Stephen C. Jacobson and Natalia Kroutchinina and Julia Khandurina and Robert S. Foote and J Michael Ramsey},
  journal={Analytical chemistry},
  year={1998},
  volume={70 1},
  pages={158-62}
}
The steps of cell lysis, multiplex PCR amplification, and electrophoretic analysis are executed sequentially on a monolithic microchip device. The entire microchip is thermally cycled to lyse cells and to amplify DNA, and the products are then analyzed using a sieving medium for size separation and an intercalating dye for fluorescence detection. Using a standard PCR protocol, a 500-base pair (bp) region of bacteriophage lambda DNA and 154-, 264-, 346-, 410-, and 550-bp regions of E. coli… CONTINUE READING

From This Paper

Topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 63 extracted citations

Similar Papers

Loading similar papers…