Microchannel wall coatings for protein separations by capillary and chip electrophoresis

  title={Microchannel wall coatings for protein separations by capillary and chip electrophoresis},
  author={Erin A. S. Doherty and Robert J. Meagher and Methal Albarghouthi and Annelise E Barron},
The necessity for microchannel wall coatings in capillary and chip‐based electrophoretic analysis of biomolecules is well understood. The regulation or elimination of EOF and the prevention of analyte adsorption is essential for the rapid, efficient separation of proteins and DNA within microchannels. Microchannel wall coatings and other wall modifications are especially critical for protein separations, both in fused‐silica capillaries, and in glass or polymeric microfluidic devices. In this… 

Permanent surface modification of polymeric capillary electrophoresis microchips for protein and peptide analysis

The structural complexity of proteins as it relates to adsorption is described, followed by a review of the mechanisms and structural characteristics of protein‐resistant surfaces.

Assessing Stability, Durability, and Protein Adsorption Behavior ofHydrophilic Silane Coatings in Glass Microchannels

Five commonly employed surface coatings are compared, and it is found that MPEG is an effective surface coating for applications where long term stability is critical, and for separation experiments, where the channel is used shortly after coating, a silanized zwitterionic sultone has superior anti-fouling characteristics.

Highly charged polyelectrolyte coatings to prevent adsorption during protein and peptide analysis in capillary electrophoresis.

This work has studied in a systematic manner the effect of coating conditions on the stability and efficiency of multilayer coatings using poly(diallyldimethylammonium) chloride (PDADMAC) as polycation and polystyrene sulfonate (PSS) aspolyanion.


Polymerized bis-SorbPC coatings have significantly longer stability against degradation and can be stored for > one year with improved separation efficiency of proteins over bare capillary separations.

Electroosmotic flow suppression in capillary electrophoresis: Chemisorption of trimethoxy silane‐modified polydimethylacrylamide

It is established that physisorption of polymer chains to the surface is essential for close alignment of surface and polymer silane groups which facilitates the formation of covalent bonds.

Novel diazoresin/carboxymethyl chitosan capillary coating for the analysis of proteins by capillary electrophoresis

A new method to prepare covalent connection capillary electrophoretic coatings is proposed and seems to be an environmental and simple way to prepare the covalently coated capillaries for CE.

Influence of polyelectrolyte capillary coating conditions on protein analysis in CE

The influence of polyelectrolyte concentration and ionic strength of the coating solutions, and the number of coating layers on coating stability and performance in limiting protein adsorption was carried out.

Wall coating for capillary electrophoresis on microchips

  • V. Dolnik
  • Materials Science
  • 2004
This review article with 116 references describes recent developments in the preparation of wall coatings for capillary electrophoresis (CE) on a microchip and concentrates on the most frequently used microchip materials.



Polyvinyl Alcohol (PVA)-Based Covalently Bonded Hydrophilic Coating for Capillary Electrophoresis

  • Northeastern University,
  • 1997

Organic Chemistry, Worth Publishers, New York

  • 1980