Microcalorimetric study on conformational change of denatured RNase A adsorbed onto a moderately hydrophobic surface

@article{Chai2010MicrocalorimetricSO,
  title={Microcalorimetric study on conformational change of denatured RNase A adsorbed onto a moderately hydrophobic surface},
  author={Y. Chai and X. Geng and A. Liu and Changzheng Zheng and Q. Bai},
  journal={Colloids and Surfaces A: Physicochemical and Engineering Aspects},
  year={2010},
  volume={361},
  pages={1-6}
}
  • Y. Chai, X. Geng, +2 authors Q. Bai
  • Published 2010
  • Chemistry
  • Colloids and Surfaces A: Physicochemical and Engineering Aspects
Abstract The microcalorimetric method was used to measure the displacement adsorption heat of denatured (by 1.8 mol L−1 guanidine hydrochloride (GuHCl)) Ribonuclease A (RNase A) adsorbed onto a moderately hydrophobic surface (PEG-600) from various ammonium sulfate ((NH4)2SO4) concentration solutions at 298 K. According to the thermodynamics of the stoichiometric displacement theory for adsorption (SDT-A) and the measured adsorption isotherms, the adsorption thermodynamic functions, ΔG, ΔS, ΔH… Expand
3 Citations
Microcalorimetric study of the adsorption of lactoferrin in supermacroporous continuous cryogel with immobilized Cu(2+) ions.
TLDR
The adsorption affinity of lactoferrin from whey in monolithic supermacroporous cryogel was analyzed using equilibrium data adsorptive isothermal titration microcalorimetry to measure thermodynamic information governing the process to enable the improvement of technical processes for the affinity separation of proteins. Expand
Pegylated species separation through an innovative PEG-grafted agarose-based resin, association quantified by microcalorimetry
Abstract The study of thermodynamic parameters of hydrophobic interaction chromatography (HIC) leads to a better understanding of protein-stationary phase interactions to design and validate newExpand
Predicting the Effect of the Homogenization Pressure on Emulsion Drop-Size Distributions
We have previously developed a population balance equation (PBE) model for emulsion drop breakage in a high-pressure homogenizer that incorporated multiple-drop formation within two mechanisms ofExpand

References

SHOWING 1-10 OF 25 REFERENCES
Microcalorimetric determination of displacement adsorption enthalpies of protein refolding on a moderately hydrophobic surface at 308 K
AbstractBoth microcalorimetric determination of displacement adsorption enthalpies ΔH and measurement of adsorbed amounts of guanidine – denatured lysozyme (Lys) refolding on the surface ofExpand
Thermodynamic analysis of denatured lysozyme folded on moderately hydrophobic surface at 298 K
Both calorimetric determination of displacement adsorption enthalpies ΔH and measurement of adsorbed amounts of lysozyme (Lyz) denatured by 1.8 mol L−1 guanidine hydrochloride (GuHCl) on a moderatelyExpand
Effect of salt concentrations on the displacement adsorption enthalpies of denatured protein folding at a moderately hydrophobic surface
AbstractThe displacement adsorption enthalpies (ΔH) of the refolding of lysozyme (Lys) denatured by 1.8 mol L–1 guanidine hydrochloride (GuHCl) on a moderately hydrophobic surface at 298 K, pH 7.0Expand
Isothermal titration microcalorimetric studies of the effect of salt concentrations in the interaction between proteins and hydrophobic adsorbents
Abstract The effect of (NH4)2SO4 concentrations on the interaction mechanism between myoglobin and two hydrophobic adsorbents, butyl- and octyl-Sepharose was investigated by the equilibrium bindingExpand
Structures and Stabilities of Adsorbed Proteins
Structural perturbations and thermodynamic-stability changes in two similar-sized globular proteins, hen egg-white lysozyme and bovine milk α-lactalbumin, upon physical adsorption to eitherExpand
Adsorption-induced conformational changes of proteins onto ceramic particles: differential scanning calorimetry and FTIR analysis.
TLDR
The two techniques applied in the present study -- micro-DSC and FTIR spectroscopy -- can be concluded to provide complementary information on adsorption-induced structural changes on both the molecular and sub-molecular level (secondary structure). Expand
Thermodynamic analysis of lysozyme adsorbed to silica.
The structural stability of hen egg white lysozyme in solution and adsorbed to small colloidal silica particles at various surface concentrations was investigated using hydrogen-deuterium (H/D)Expand
Fractions of thermodynamic functions for native lysozyme adsorption onto moderately hydrophobic surface
Calorimetric measurement of adsorption enthalpies of native lysozyme(Lyz) on a moderately hydrophobic surface at 25°C, pH 7.0 and various salt concentrations was performed. Based on theExpand
Incorporation of styrene enhances recognition of ribonuclease A by molecularly imprinted polymers.
Ribonuclease A (RNase A) is an RNA-cleaving enzyme characterized by its high conformational stability and strong catalytic activity. This enzyme is ubiquitous in living organisms and is difficult toExpand
Calorimetric determination of enthalpies of lysozyme folding at a liquid-solid interface
SummaryCalorimetric determination of the total enthalpy changes (ΔHi) of guanidine-denatured lysozyme (Lys) during the adsorption with simultaneously refolding on the surface of hydrophobicExpand
...
1
2
3
...