Microbiological analysis of 5-formyltetrahydrofolic acid and other folates using an automatic 96-well plate reader.


The growth of auxotrophic bacteria remains the method of choice for the determination of biologically active folate metabolites in plasma. This report describes a microbiological assay for folates adapted to use disposable 96-well plates and an automatic plate reader. The modifications in the assay decreased reagent costs and made the analysis of hundreds… (More)


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