Microbial oxidation of methanol: purification and properties of formaldehyde dehydrogenase from a Pichia sp. NRRL-Y-11328.

@article{Patel1983MicrobialOO,
  title={Microbial oxidation of methanol: purification and properties of formaldehyde dehydrogenase from a Pichia sp. NRRL-Y-11328.},
  author={Ramesh N. Patel and Ching Tsang Hou and Patricia Derelanko},
  journal={Archives of biochemistry and biophysics},
  year={1983},
  volume={221 1},
  pages={
          135-42
        }
}
Abstract An NAD+-linked, reduced glutathione-dependent formaldehyde dehydrogenase was purified to homogeneity from soluble extracts of methanol-grown yeast, Pichia sp. Formaldehyde and methylglyoxal are oxidized in the presence of NAD+ as an electron acceptor. NADP+ could not replace NAD+. Other straight chain aldehydes (C2–C6 tested), branched-chain aldehydes (e.g., isobutyaldehyde), aromatic aldehydes (e.g., salicylal-dehyde, benzaldehyde), glutyraldehyde, glyceraldehyde, glycoaldehyde, and… CONTINUE READING