Microbial nitrilases: versatile, spiral forming, industrial enzymes.

  title={Microbial nitrilases: versatile, spiral forming, industrial enzymes.},
  author={Robert N. Thuku and Declan Brady and Michael J. Benedik and B. Trevor Sewell},
  journal={Journal of applied microbiology},
  volume={106 3},
The nitrilases are enzymes that convert nitriles to the corresponding acid and ammonia. They are members of a superfamily, which includes amidases and occur in both prokaryotes and eukaryotes. The superfamily is characterized by having a homodimeric building block with a alpha beta beta alpha-alpha beta beta alpha sandwich fold and an active site containing four positionally conserved residues: cys, glu, glu and lys. Their high chemical specificity and frequent enantioselectivity makes them… CONTINUE READING

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