Microbial nitrilases: versatile, spiral forming, industrial enzymes.

@article{Thuku2009MicrobialNV,
  title={Microbial nitrilases: versatile, spiral forming, industrial enzymes.},
  author={Robert N. Thuku and Declan Brady and Michael J. Benedik and B. Trevor Sewell},
  journal={Journal of applied microbiology},
  year={2009},
  volume={106 3},
  pages={703-27}
}
The nitrilases are enzymes that convert nitriles to the corresponding acid and ammonia. They are members of a superfamily, which includes amidases and occur in both prokaryotes and eukaryotes. The superfamily is characterized by having a homodimeric building block with a alpha beta beta alpha-alpha beta beta alpha sandwich fold and an active site containing four positionally conserved residues: cys, glu, glu and lys. Their high chemical specificity and frequent enantioselectivity makes them… CONTINUE READING

From This Paper

Figures, tables, and topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 32 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 74 references

aliphatic amidase from Geobacillus pallidus RAPc8

C. Kiziak, D. Conradt, A. Stolz, R. Mattes, J. Klein
Acta Crystallogr Sect D: Biol Crystallogr • 2005
View 13 Excerpts
Highly Influenced

cyanide degrading nitrilase from Pseudomonas stutzeri AK61 is a two-fold symmetric, 14subunit spiral

Sewell, B.T, R. N. Thuku, X. Zhang, M. J. Benedik
Ann NY Acad Sci • 2005
View 20 Excerpts
Highly Influenced

Mechanistic and structural studies on Rhodococcus ATCC 39484 nitrilase.

Biotechnology and applied biochemistry • 1992
View 7 Excerpts
Highly Influenced

Influence of different carboxy-terminal mutations on the substrate-, reaction- and enantiospecificity of the arylacetonitrilase from Pseudomonas fluorescens

C. Kiziak, J. Klein, A. Stoltz
EBC191.Prot Eng Des Sel 20, • 2007
View 9 Excerpts
Highly Influenced

Nitrile hydratase activity of a recombinant nitrilase

B.C.M. Fernandes, C. Mateo, +5 authors Sheldon R.A
Adv Synth Catal 348, • 2006
View 4 Excerpts
Highly Influenced

hydratase enzyme of Fusarium lateritium also has nitrilase activity

C. O’Reilly, P. D. Turner
FEMS Microbiol Lett 221, • 2003
View 5 Excerpts
Highly Influenced

applications of recombinant AtNIT1 from Arabidopsis thaliana

H. C. Pace, S. C. Hodawadekar, +5 authors C. Brenner
Eur J Biochem • 2000
View 8 Excerpts
Highly Influenced

Similar Papers

Loading similar papers…