Microbial hydrogenases: primary structure, classification, signatures and phylogeny.

@article{Wu1993MicrobialHP,
  title={Microbial hydrogenases: primary structure, classification, signatures and phylogeny.},
  author={L. F. Wu and M A Mandrand},
  journal={FEMS microbiology reviews},
  year={1993},
  volume={10 3-4},
  pages={
          243-69
        }
}
  • L. Wu, M. Mandrand
  • Published 1 April 1993
  • Biology, Medicine
  • FEMS microbiology reviews
Thirty sequenced microbial hydrogenases are classified into six classes according to sequence homologies, metal content and physiological function. The first class contains nine H2-uptake membrane-bound NiFe-hydrogenases from eight aerobic, facultative anaerobic and anaerobic bacteria. The second comprises four periplasmic and two membrane-bound H2-uptake NiFe(Se)-hydrogenases from sulphate-reducing bacteria. The third consists of four periplasmic Fe-hydrogenases from strict anaerobic bacteria… 
Classification and phylogeny of hydrogenases.
TLDR
Compelling evidence from sequences and structures indicates that the [NiFe]- and [Fe]-H2ases are phylogenetically distinct classes of proteins, which would be consistent with the phylogenetic distinctiveness of the two classes of H2ases.
Genomic and metagenomic surveys of hydrogenase distribution indicate H2 is a widely utilised energy source for microbial growth and survival
TLDR
It is predicted that this hydrogenase diversity supports H2-based respiration, fermentation and carbon fixation processes in both oxic and anoxic environments, in addition to various H1N1-sensing, electron-bifurcation and energy-conversion mechanisms.
[NiFe] hydrogenases from the hyperthermophilic bacterium Aquifex aeolicus: properties, function, and phylogenetics
TLDR
It is demonstrated that all three hydrogenases are expressed under standard growth conditions of the organism and a model for the metabolic roles of the three enzymes is proposed on the basis of the obtained results.
The Model [NiFe]-Hydrogenases of Escherichia coli.
  • F. Sargent
  • Biology, Medicine
    Advances in microbial physiology
  • 2016
TLDR
The versatile hydrogen metabolism of E. coli will be discussed and the roles and potential applications of the spectrum of different types of [NiFe]-hydrogenases available will be explored.
EIGHT The Model [ NiFe ]-Hydrogenases of Escherichia coli
In Escherichia coli, hydrogenmetabolism plays a prominent role in anaerobic physiology. The genome contains the capability to produce and assemble up to four [NiFe]-hydrogenases, each of which are
Cyanobacterial-Type, Heteropentameric, NAD+-Reducing NiFe Hydrogenase in the Purple Sulfur Photosynthetic Bacterium Thiocapsa roseopersicina
TLDR
In-frame deletion of the hoxE gene eliminated hydrogen evolution derived from the Hox enzyme in vivo, although it had no effect on the hydrogenase activity in vitro, suggesting that HoxE has a hydrogenase-related role; it likely participates in the electron transfer processes.
Novel approaches to exploit microbial hydrogen metabolism
Publisher Summary The key enzyme in biological hydrogen (H2) metabolism is hydrogenase, which catalyzes the formation and decomposition of H2. Hydrogenases are metalloenzymes harboring Ni and Fe, or
Molecular biology of microbial hydrogenases.
TLDR
Recent advances that have greatly increased knowledge of microbial H2ases are emphasised, including the structure of their active site, how the metallocenters are synthesized and assembled, how they function,How the synthesis of these enzymes is controlled by external signals, and their potential use in biological H2 production.
The [NiFe] hydrogenases of Methanococcus voltae: genes, enzymes and regulation
TLDR
Methanococcus voltae carries genetic information for four [NiFe] hydrogenases, which are linked by an intergenic region in which regulatory cis elements were defined by employing reporter gene constructs and site-directed mutagenesis.
Molecular biological analysis of a bidirectional hydrogenase from cyanobacteria.
TLDR
An 8.9-kb segment with hydrogenase genes from the cyanobacterium Anabaena variabilis has been cloned and sequenced, corroborating the molecular biological demonstration of the NAD(P)(+)-dependent hydrogenase in cyanobacteria.
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 109 REFERENCES
The three classes of hydrogenases from sulfate-reducing bacteria of the genus Desulfovibrio.
TLDR
Three types of hydrogenases have been isolated from the sulfate-reducing bacteria of the genus Desulfovibrio and it is suggested that selenium is a ligand to nickel and suggest that the redox active nickel is ligated by at least two cysteinyl thiolate and one selenocysteine selenolate residues.
Primary structure of hydrogenase I from Clostridium pasteurianum.
TLDR
Comparisons of the available [Fe] hydrogenase sequences show that these enzymes constitute a structurally rather homogeneous family, and show that the H clusters most probably possess a common structural framework in all [Fe], which possibly contain six to eight iron atoms.
Molecular biology studies of the uptake hydrogenase of Rhodobacter capsulatus and Rhodocyclus gelatinosus.
TLDR
The deduced protein sequences of the small and of the large subunits share nearly 80% and maximally 70% identity, respectively, with their counterparts in uptake hydrogenases found in N2-fixing bacteria.
Structure-function relationships among the nickel-containing hydrogenases.
TLDR
It is proposed that nickel is ligated solely by amino acid residues of the large subunit and that the non-heme iron clusters are ligated by other cysteine-rich polypeptides encoded in the hydrogenase operons which are not necessarily homologous in either structure or function.
Cloning and sequencing of a [NiFe] hydrogenase operon from Desulfovibrio vulgaris Miyazaki F.
TLDR
The subunit molecular masses and amino acid composition derived from the gene sequence are very similar to the data reported for the periplasmic, membrane-bound hydrogenase isolated by Yagi and coworkers, suggesting that this hydrogenase belongs to the general class of [NiFe] hydrogenases, despite its low nickel content and apparently anomalous spectral properties.
The structure and mechanism of iron-hydrogenases.
  • M. Adams
  • Chemistry, Medicine
    Biochimica et biophysica acta
  • 1990
TLDR
Mechanisms of H2 activation and electron transfer are proposed to explain the effects of CO binding and the ability of one of the hydrogenases to preferentially catalyze H2 oxidation and H2 production.
Hydrogenase, nitrogenase, and hydrogen metabolism in the photosynthetic bacteria.
TLDR
This chapter illustrates the H 2 metabolism in photosynthetic bacteria with developments in the context of the biochemistry and physiology of the photosynthesis bacteria, and their biotechnological applications.
Soluble hydrogenase of Anabaena cylindrica
A gene potentially encoding a subunit of the soluble hydrogenase of Anabaena cylindrica was isolated from a genomic library by screening with a set of redundant oligonucleotides, the sequence of
FORMIC DEHYDROGENASE AND THE HYDROGENLYASE ENZYME COMPLEX IN COLI-AEROGENES BACTERIA
TLDR
Investigation of the enzymatic constitution of six coli-aerogenes bacteria (anaerogenic variants) incapable of producing hydrogen from glucose found cell-free extracts of one of the variants catalyzed the hydrogenlyase reaction and showed both formic dehydrogenase and hydrogenase activities.
Immunological and molecular evidence for a membrane-bound, dimeric hydrogenase in Rhodopseudomonas capsulata
TLDR
It is concluded that the photosynthetic microorganism, R. capsulata, contains a membranes-bound, dimeric hydrogenase that is immunologically related to the membrane-bound hydrogenase from the aerobic microorganisms mentioned.
...
1
2
3
4
5
...