Microbial gene expression in soil: methods, applications and challenges.

@article{SalehLakha2005MicrobialGE,
  title={Microbial gene expression in soil: methods, applications and challenges.},
  author={Saleema Saleh-Lakha and Michelle Susan Miller and Robert Gordon Campbell and Kim N. Schneider and Parastu Elahimanesh and Miranda M Hart and J. T. Trevors},
  journal={Journal of microbiological methods},
  year={2005},
  volume={63 1},
  pages={
          1-19
        }
}
About 99% of soil microorganisms are unculturable. However, advances in molecular biology techniques allow for the analysis of living microorganisms. With the advent of new technologies and the optimization of previous methods, various approaches to studying gene expression are expanding the field of microbiology and molecular biology. Methods used for RNA extraction, DNA microarrays, real-time PCR, competitive RT-PCR, stable isotope probing and the use of reporter genes provide methods for… CONTINUE READING
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