Microbial engineering for the production of advanced biofuels

  title={Microbial engineering for the production of advanced biofuels},
  author={Pamela Peralta-Yahya and Fuzhong Zhang and Stephen B. del Cardayr{\'e} and Jay D. Keasling},
Advanced biofuels produced by microorganisms have similar properties to petroleum-based fuels, and can 'drop in' to the existing transportation infrastructure. However, producing these biofuels in yields high enough to be useful requires the engineering of the microorganism's metabolism. Such engineering is not based on just one specific feedstock or host organism. Data-driven and synthetic-biology approaches can be used to optimize both the host and pathways to maximize fuel production… 

Production of advanced biofuels in engineered E. coli.

Next generation biofuel engineering in prokaryotes.

Metabolic engineering for isoprenoid‐based biofuel production

The engineered isoprenoid biosynthetic pathways in well‐characterized microbial systems for the production of several isopranoid‐based biofuels and fuel precursors are described.

Biofuels Production Using Metabolic Engineering

This chapter gives brief insight into different strategies and techniques conferring metabolic engineering and highlights the challenges on more advanced level.

Microbial pathways for advanced biofuel production

  • John Love
  • Engineering, Environmental Science
    Biochemical Society transactions
  • 2022
Decarbonisation of the transport sector is essential to mitigate anthropogenic climate change. Microbial metabolisms are already integral to the production of renewable, sustainable fuels and,

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There is justified optimism that the full potential of biofuel production from cellulosic biomass will be obtainable in the next 10 to 15 years.

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The four major metabolic systems, the coenzyme-A mediated pathways, the keto acid pathways,The fatty acid pathway, and the isoprenoid pathways, that allow production of these fuel-grade chemicals are reviewed.

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The first production of isobutanol to approximately 660 mg/liter from crystalline cellulose is demonstrated by using this microorganism, which exploits the host's natural cellulolytic activity and the amino acid biosynthesis pathway and diverts its 2-keto acid intermediates toward alcohol synthesis.

Identification and microbial production of a terpene-based advanced biofuel

A framework for the identification of novel terpene-based advanced biofuels and the rapid engineering of microbial farnesyl diphosphate-overproducing platforms for the production of bio Fuels is presented.