Microbial dark matter ecogenomics reveals complex synergistic networks in a methanogenic bioreactor

  title={Microbial dark matter ecogenomics reveals complex synergistic networks in a methanogenic bioreactor},
  author={Masaru Konishi Nobu and Takashi Narihiro and Christian Rinke and Yoichi Kamagata and Susannah G. Tringe and Tanja Woyke and Wen-Tso Liu},
  journal={The ISME Journal},
Ecogenomic investigation of a methanogenic bioreactor degrading terephthalate (TA) allowed elucidation of complex synergistic networks of uncultivated microorganisms, including those from candidate phyla with no cultivated representatives. Our previous metagenomic investigation proposed that Pelotomaculum and methanogens may interact with uncultivated organisms to degrade TA; however, many members of the community remained unaddressed because of past technological limitations. In further… 
Quantitative Metaproteomics Highlight the Metabolic Contributions of Uncultured Phylotypes in a Thermophilic Anaerobic Digester
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Catabolism and interactions of uncultured organisms shaped by eco-thermodynamics in methanogenic bioprocesses
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Metabolic strategies of marine subseafloor Chloroflexi inferred from genome reconstructions.
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Bioreactor microbial ecosystems with differentiated methanogenic phenol biodegradation and competitive metabolic pathways unraveled with genome-resolved metagenomics
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Syntrophic propionate-oxidizing bacteria in methanogenic systems
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Concerted Metabolic Shifts Give New Insights Into the Syntrophic Mechanism Between Propionate-Fermenting Pelotomaculum thermopropionicum and Hydrogenotrophic Methanocella conradii
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Genome-Guided Analysis and Whole Transcriptome Profiling of the Mesophilic Syntrophic Acetate Oxidising Bacterium Syntrophaceticus schinkii
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Fermentative Spirochaetes mediate necromass recycling in anoxic hydrocarbon-contaminated habitats
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Multiple syntrophic interactions in a terephthalate-degrading methanogenic consortium
Observations suggest that the TA-degrading consortium consists of additional syntrophic interactions beyond the standard H2-producing syntroph–methanogen partnership that may serve to improve community stability.
Community and Proteomic Analysis of Methanogenic Consortia Degrading Terephthalate
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The genome of Pelotomaculum thermopropionicum reveals niche-associated evolution in anaerobic microbiota.
Genes for most of the important catabolic enzymes are physically linked to those for PAS-domain-containing regulators, suggesting that the catabolic pathways are regulated in response to environmental conditions and/or global cellular situations rather than specific substrates.
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Microbial community structure in a thermophilic anaerobic hybrid reactor degrading terephthalate.
Results suggested that Methanothrix thermophila-related methanogens, Desulfotomaculum-related bacterial populations in the Gram-positive low-G + C group, and OP5-related populations were the key members responsible for terephthalate degradation under thermophilic methanogenic conditions except during periods when the reactor experienced heat shock and pump failure.