Microbial community analysis of a methane-oxidizing biofilm using ribosomal tag pyrosequencing.

@article{Kim2012MicrobialCA,
  title={Microbial community analysis of a methane-oxidizing biofilm using ribosomal tag pyrosequencing.},
  author={Tae Gwan Kim and Eun-Hee Lee and Kyung-Suk Cho},
  journal={Journal of microbiology and biotechnology},
  year={2012},
  volume={22 3},
  pages={
          360-70
        }
}
Current ecological knowledge of methanotrophic biofilms is incomplete, although they have been broadly studied in biotechnological processes. Four individual DNA samples were prepared from a methanotrophic biofilm, and a multiplex 16S rDNA pyrosequencing was performed. A complete library (before being de-multiplexed) contained 33,639 sequences (average length, 415 nt). Interestingly, methanotrophs were not dominant, only making up 23% of the community. Methylosinus, Methylomonas, and… CONTINUE READING

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