Microbial asymmetric reduction of ethyl 4-chloro-3-oxobutanoate to optically active ethyl-4-chloro-3-hydroxybutanoate

@article{Shimizu2005MicrobialAR,
  title={Microbial asymmetric reduction of ethyl 4-chloro-3-oxobutanoate to optically active ethyl-4-chloro-3-hydroxybutanoate},
  author={Seishi Shimizu and Masakazu Kataoka and Atsuki Morishita and Misugi Katoh and Tomoyuki Morikawa and Teruzo Miyoshi and Hidetoshi Yamada},
  journal={Biotechnology Letters},
  year={2005},
  volume={12},
  pages={593-596}
}
The synthesis of ethyl (R)-4-chloro-3-hydroxybutanoate through the asymmetric reduction of ethyl 4-chloro-3-oxobutanoate with the NADPH-dependent aldehyde reductase ofSporobolomyces salmonicolor AKU 4429 is described. Under preparative scale reaction conditions with the acetone-fractionated aldehyde reductase, the amount of ethyl-4-chloro-3-hydroxybutanoate reached 33.1 mg/ml (85%ee; molar yield, 74.0%). Furthermore, conversion to ethyl (S)-4-chloro-3-hydroxybutanoate occurred on incubation… CONTINUE READING

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