Microbial Tyrosinases: A Novel Enzyme, Structural Features, and Applications

@article{Agarwal2019MicrobialTA,
  title={Microbial Tyrosinases: A Novel Enzyme, Structural Features, and Applications},
  author={Pragati Agarwal and Mukta Singh and Jyoti Singh and R.P. Singh},
  journal={Applied Microbiology and Bioengineering},
  year={2019}
}
Considerations Regarding Activity Determinants of Fungal Polyphenol Oxidases Based on Mutational and Structural Studies
TLDR
The crystal structure of a TtPPO variant, determined at 1.55 Å resolution, represents the second known structure of an ascomycete PPO and improves current understanding of structure-function relations of microbial PPOs, which is a prerequisite for the engineering of biocatalysts of desired properties.
New tyrosinases with a broad spectrum of action against contaminants of emerging concern: Insights from in silico analyses
TLDR
Ciliates, including those caring tyrosinase genes within their genomes are fast-growing unicellular microeukaryotes that can be efficiently culturable at large-scales under in vitro conditions, should be regarded as potential low-cost sources for the production of relevant biotechnological molecules.
New tyrosinases with putative action against contaminants of emerging concern
TLDR
Six tyrosinase homologs were identified and characterized from the genomes of four widespread freshwater ciliates using bioinformatics and a virtual screening was performed to calculate binding energies between 3D models of these homologicals and contaminants of emerging concern (CECs), as an indirect approach to identify likely and unlikely targets for tyosinases.
A Novel Tyrosinase from Armillaria ostoyae with Comparable Monophenolase and Diphenolase Activities Suffers Substrate Inhibition
TLDR
The results indicated that this novel tyrosinase from Armillaria ostoyaestrain C18/9 (AoTyr) could be a potential candidate for the industrial biosynthesis of catechols.
Effectivity of tyrosinase purification by membrane techniques versus fractionation by salting out
TLDR
It was shown that tyrosinase can be pre-purified by selected membranes yielding the enzyme quality at least comparable to that after double salting-out method but in one device.

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