Microbial Biofilms on the Sandstone Monuments of the Angkor Wat Complex, Cambodia

  title={Microbial Biofilms on the Sandstone Monuments of the Angkor Wat Complex, Cambodia},
  author={Christine C. Gaylarde and C{\'e}sar Rodr{\'i}guez and Yendi E. Navarro-Noya and Benjam{\'i}n Otto Ortega-Morales},
  journal={Current Microbiology},
Key ResultDiscoloring biofilms from Cambodian temples Angkor Wat, Preah Khan, and the Bayon and West Prasat in Angkor Thom contained a microbial community dominated by coccoid cyanobacteria. Molecular analysis identified Chroococcidiopsis as major colonizer, but low similarity values (<95%) suggested a similar genus or species not present in the databases.

Microbiomes of Biofilms on Decorative Siliceous Stone: Drawbacks and Advantages of Next Generation Sequencing

Principal Components Analysis separated façade biofilms into the appropriate three groups and indicated greater dissimilarity of the tree-surrounded church biofilm from the others, confirmed by Jaccard Similarity coefficients, suggesting that local environment influences community composition more than stone type.

Molecular diversity of phototrophic biofilms on building stone.

A comparative study addressing the composition of algal communities on sandstone substrata based upon the analysis of rRNA gene clone libraries from environmental samples and crude cultures finds endolithic algal morphotypes (coccoid algae and diatoms) dominant in open pores between mineral particles.

Fungal communities in the biofilms colonizing the basalt sculptures of the Leizhou Stone Dogs and assessment of a conservation measure

The findings provide an overview of fungi diversity in the biofilms on the stone dogs and help the investigation of fungi-induced biodeterioration and the exploration of specific conservation measure.

Cryptogam covers on sepulchral monuments and re-colonization of a marble surface after cleaning

A comparative study addressing the composition of algal and fungal communities on a marble sculpture, based upon the analysis of 18S rRNA gene clone libraries from environmental samples, finds the green algal community was dominated by different phylotypes of the lichen algae Trebouxia, as well as the cosmopolitan green algae Apatococcus and Stichococcus.

Biofilm architecture on different substrates of an Oculatella subterranea (Cyanobacteria) strain isolated from Pompeii archaeological site (Italy)

The results show that the three-dimensional structure of O. subterranea microbial mats is poorly affected by physical and geochemical features of substrates: in fact, the porous architecture of its biofilm was preserved, independently of the materials.

Diversity of Biodeteriorative Bacterial and Fungal Consortia in Winter and Summer on Historical Sandstone of the Northern Pergola, Museum of King John III’s Palace at Wilanow, Poland

The aim of the presented investigation was to describe seasonal changes of microbial community composition in situ in different biocenoses on historical sandstone of the Northern Pergola in the

Depth profiles of microbial colonization in sandstones

This feature may imply stronger impact of stone decay induced by endolithic growth of bryophytes than hitherto observed and contribute to an increase in pore size by active penetration of the clastic material, even though colonization of the surface by mosses may not be necessarily obvious.

Cyanobacteria cause black staining of the National Museum of the American Indian Building, Washington, DC, USA

Observations indicated that biofilms, which penetrated up to a maximum depth of about 1 mm, were mainly composed of cyanobacteria, with the predominance of Gloeocapsa and Lyngbya, while denaturing gradient gel electrophoresis analysis revealed that the microbial community also included eukaryotic algae and fungi, along with a consortium of bacteria.



Microbial Community Analysis of Fresh and Old Microbial Biofilms on Bayon Temple Sandstone of Angkor Thom, Cambodia

A comparison of the microbial communities between the fresh and old biofilms revealed that the bacterial community of old biofilm was very similar to the newly formed fresh biofilm in terms of bacterial composition, but the eukaryotic communities were distinctly different between these two.

Biofilms fouling ancient limestone Mayan monuments in Uxmal, Mexico: a cultivation-independent analysis

Biofilms colonizing ancient limestone Mayan monuments in Uxmal (Yucatan, Mexico) were characterized for their microbial composition and differences using phospholipid fatty acid (PLFA) analysis and

Phototrophic Biofilms on Ancient Mayan Buildings in Yucatan, Mexico

It is shown that phototrophic biofilms may contribute to biodegradation by providing nutrients that support growth of acid-producing fungi and bacteria and active “boring” behavior, the solubilized calcium being reprecipitated as calcium carbonate.

Lichen-like colonies of pure Trentepohlia on limestone monuments

Biogenic Black Crusts on Buildings in Unpolluted Environments

Analysis of peeling black crusts from modern and historic buildings in Campeche, Mexico, from a gravestone on the island of Dom Khon, Lao, and from the Anglican cathedral in Belize City demonstrate that, unlike chemically formed thickBlack crusts found in polluted atmospheres, thinblack crusts in clean environments may be predominantly composed of filamentous cyanobacteria.

Epilithic and Endolithic Bacterial Communities in Limestone from a Maya Archaeological Site

A large endolithic bacterial community in limestone from the interior of the Maya archaeological site Ek' Balam is found and the presence of differing epilithic and endolithicacterial communities may be a significant factor for conservation of stone cultural heritage materials and quantitative prediction of carbonate weathering.

Paenibacillus sepulcri sp. nov., isolated from biodeteriorated mural paintings in the Servilia tomb.

The isolate represents a novel species, for which the name Paenibacillus sepulcri sp.

Polyphasic Detection of Cyanobacteria in Terrestrial Biofilms

The polyphasic strategy described here was to select morphologically distinct colonies from rehydrated biofilms for direct DNA amplification, allowing uncultured organisms to be sequenced and their morphology to be characterized by microscopy.

Bacterial diversity in biofilms on external surfaces of historic buildings in Porto Alegre

Gram-positive bacteria, although present in lower numbers, are principally responsible for the damage caused, and the isolates with the highest deteriorating ability, producing acids and surfactants with autoemulsifing power were Bacillus isolates B4, B6 and B10 from Priest groups II, I and III.