Microbead display by in vitro compartmentalisation: selection for binding using flow cytometry.

@article{Sepp2002MicrobeadDB,
  title={Microbead display by in vitro compartmentalisation: selection for binding using flow cytometry.},
  author={Armin Sepp and Dan S. Tawfik and Andrew D. Griffiths},
  journal={FEBS letters},
  year={2002},
  volume={532 3},
  pages={455-8}
}
In vitro compartmentalisation in an emulsion was used to physically link proteins to the DNA that encodes them via microbeads. These microbeads can be selected for catalysis, or, as demonstrated here, for binding. Genes encoding a peptide containing an epitope (haemagglutinin) were enriched to near purity from a 10(6)-fold excess of genes encoding a different peptide by two rounds of selection using flow cytometry, indicating approximately 1000-fold enrichment per round. Single beads can be… CONTINUE READING