Microassay for proteins on nitrocellulose filter using protein dye-staining procedure.

A simple and rapid microassay for proteins utilizing the protein dye-staining procedure with a nitrocellulose (NC) filter is described. Proteins were directly bound to an NC filter using the "BIO DOT" microfiltration apparatus to ensure their uniformity. The proteins were then stained with a dye (Ponceau Red 3R or amido black 10B), and the optical density… CONTINUE READING