Microarrays of tumor cell derived proteins uncover a distinct pattern of prostate cancer serum immunoreactivity.

@article{Bouwman2003MicroarraysOT,
  title={Microarrays of tumor cell derived proteins uncover a distinct pattern of prostate cancer serum immunoreactivity.},
  author={Kerri Bouwman and Ji Yan Qiu and Heping Zhou and Mark P Schotanus and Leslie A. Mangold and Robert C. Vogt and Erik J. Erlandson and John M. Trenkle and Alan W. Partin and David E. Misek and Gilbert S. Omenn and Brian B. Haab and Samir M Hanash},
  journal={Proteomics},
  year={2003},
  volume={3 11},
  pages={2200-7}
}
The broad characterization of the immune responses elicited by tumors has valuable applications in diagnostics and basic research. We present here the use of microarrays of tumor-derived proteins to profile the antibody repertoire in the sera of prostate cancer patients and controls. Two-dimensional liquid chromatography was used to separate proteins from the prostate cancer cell line LNCaP into 1760 fractions. These fractions were spotted in microarrays on coated microscope slides, and the… CONTINUE READING
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