Microarrays of lentiviruses for gene function screens in immortalized and primary cells

@article{Bailey2006MicroarraysOL,
  title={Microarrays of lentiviruses for gene function screens in immortalized and primary cells},
  author={Steve N Bailey and Siraj M Ali and Anne E. Carpenter and Caitlin O Higgins and David M. Sabatini},
  journal={Nature Methods},
  year={2006},
  volume={3},
  pages={117-122}
}
Here we describe lentivirus-infected cell microarrays for the high-throughput screening of gene function in mammalian cells. To create these arrays, we cultured mammalian cells on glass slides 'printed' with lentiviruses pseudotyped as vesicular stomatitis virus glycoprotein, which encode short hairpin RNA or cDNA. Cells that land on the printed 'features' become infected with lentivirus, creating a living array of stably transduced cell clusters within a monolayer of uninfected cells. The… CONTINUE READING
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