Microarray for Genes Associated with Signal Transduction in Diabetic OLETF Keratocytes

@inproceedings{Lee2007MicroarrayFG,
  title={Microarray for Genes Associated with Signal Transduction in Diabetic OLETF Keratocytes},
  author={Ji-Eun Lee and Jong Soo Lee and Sang Ho Hwang},
  booktitle={Korean journal of ophthalmology : KJO},
  year={2007}
}
PURPOSE The purpose of this study was to identify differences in signal transduction gene expression between normal and diabetic keratocytes stimulated with interleukin-1alpha (IL-1alpha) and tumor necrosis factor-alpha (TNF-alpha). METHODS Normal and diabetic keratocytes were primarily cultured and treated with 20 ng/ml IL-1alpha and TNF-alpha for 6 h. cDNA was hybridized to an oligonucleotide microarray. Genes identified by the microarray were further evaluated by real-time PCR. RESULTS… CONTINUE READING

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