MicroRNA expression profiling to identify and validate reference genes for relative quantification in colorectal cancer

@inproceedings{Chang2009MicroRNAEP,
  title={MicroRNA expression profiling to identify and validate reference genes for relative quantification in colorectal cancer},
  author={Kah Hoong Chang and Pieter Mestdagh and Jo Vandesompele and Michael John Kerin and Nicola M Miller},
  booktitle={BMC Cancer},
  year={2009}
}
BackgroundAdvances in high-throughput technologies and bioinformatics have transformed gene expression profiling methodologies. The results of microarray experiments are often validated using reverse transcription quantitative PCR (RT-qPCR), which is the most sensitive and reproducible method to quantify gene expression. Appropriate normalisation of RT-qPCR data using stably expressed reference genes is critical to ensure accurate and reliable results. Mi(cro)RNA expression profiles have been… CONTINUE READING
BETA

Similar Papers

Citations

Publications citing this paper.
SHOWING 1-10 OF 123 CITATIONS, ESTIMATED 58% COVERAGE

MicroRNAome genome: a treasure for cancer diagnosis and therapy.

VIEW 6 EXCERPTS
CITES BACKGROUND
HIGHLY INFLUENCED

miRNA expressions in rectal cancer as predictors of response to neoadjuvant chemoradiation therapy

  • International Journal of Colorectal Disease
  • 2012
VIEW 7 EXCERPTS
CITES BACKGROUND
HIGHLY INFLUENCED

MicroRNA-21 and PDCD4 expression in colorectal cancer.

  • European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology
  • 2011
VIEW 3 EXCERPTS
CITES METHODS
HIGHLY INFLUENCED

FILTER CITATIONS BY YEAR

2009
2019

CITATION STATISTICS

  • 6 Highly Influenced Citations

  • Averaged 8 Citations per year from 2017 through 2019

References

Publications referenced by this paper.
SHOWING 1-10 OF 46 REFERENCES

A novel and universal method for microRNA RT-qPCR data normalization

P Mestdagh, P van Vlierberghe, +4 authors J Vandesompele
  • Genome Biol
  • 2009
VIEW 3 EXCERPTS
HIGHLY INFLUENTIAL