MicroRNA expression profiles classify human cancers

  title={MicroRNA expression profiles classify human cancers},
  author={Jun Lu and Gad Getz and Eric Alexander Miska and Ezequiel Alvarez-Saavedra and Justin Lamb and David Peck and Alejandro Sweet-Cordero and Benjamin L. Ebert and Raymond H. Mak and Adolfo A. Ferrando and James R. Downing and Tyler Jacks and H. Robert Horvitz and Todd R. Golub},
Recent work has revealed the existence of a class of small non-coding RNA species, known as microRNAs (miRNAs), which have critical functions across various biological processes. Here we use a new, bead-based flow cytometric miRNA expression profiling method to present a systematic expression analysis of 217 mammalian miRNAs from 334 samples, including multiple human cancers. The miRNA profiles are surprisingly informative, reflecting the developmental lineage and differentiation state of the… 
Expression profiling of microRNAs in cancer cells: technical considerations.
This chapter summarizes the recent findings and highlights the technical advances in miRNA probe preparation, miRNA expression profiling and target identification.
MicroRNAs and Cancer
How miRNAs function in cancers, what causes aberrant miRNA expression in cancers), and the potential in utilizing miRNAAs as biomarkers and cancer therapies are reviewed.
miRNA profiling for diagnosis and prognosis of human cancer.
The evaluation of miRNAs expression may be of prognostic value, as best exemplified by the correlation of let-7 and mir-155 levels with disease survival in nonsmall cell lung cancer.
MicroRNA expression profiling in cancer from a bioinformatics prospective
The further advancement of miRNA-detection technologies and bioinformatics algorithms is critical for successful identification of the most informative diagnostics markers among the tissue-specific miRNAs.
MicroRNAs and cancer: Profile, profile, profile
The identification of signatures associated with diagnosis, prognosis and response to treatment of human tumors and miRNAs fingerprinting represents a new addition to the tools to be used by medical oncologists.
miRNA profiling of cancer.
Circulating MicroRNA Profiling in Cancer Biomarker Discovery
This chapter analyzes the major pre-analytical and analytical challenges in experimental design for circulating miRNA detection, focusing on exosome fraction and microarray-based approach.
Informative MicroRNA Expression Patterns for Cancer Classification
Some non-coding small RNAs, known as microRNAs (miRNAs), have been shown to play important roles in gene regulation and various biological processes. The abnormal expression of some specific miRNA


An oligonucleotide microchip for genome-wide microRNA profiling in human and mouse tissues.
A recently developed methodology for miRNA gene expression profiling based on the development of a microchip containing oligonucleotides corresponding to 245 miRNAs from human and mouse genomes is described.
MicroRNA profiling reveals distinct signatures in B cell chronic lymphocytic leukemias.
Genomewide expression profiling of miRNAs in human B cell chronic lymphocytic leukemia (CLL) is reported by using a microarray containing hundreds of human precursor and mature miRNA oligonucleotide probes, suggesting that miRNA expression patterns have relevance to the biological and clinical behavior of this leukemia.
Probing microRNAs with microarrays: tissue specificity and functional inference.
It is found that the expression of target mRNAs predicted on the basis of sequence complementarity is unrelated to the tissues in which the corresponding miRNA is expressed, suggesting that miRNAs have widespread regulatory roles in adults.
Microarray-based, high-throughput gene expression profiling of microRNAs
It is demonstrated that miRNAs can be isolated and profiled from formalin-fixed paraffin-embedded tissue, which opens up new opportunities for analyses of small RNAs from archival human tissue.
A custom microarray platform for analysis of microRNA gene expression
Dual-channel microarrays are designed that monitor expression levels of 124 mammalian microRNAs and expression profiles of staged embryos demonstrate temporal regulation of a large class of micro RNAs, including members of the let-7 family.
Reduced accumulation of specific microRNAs in colorectal neoplasia.
A study was undertaken to investigate possible changes in microRNA levels during tumorigenesis, identifying a total of 28 different miRNA sequences, including 3 novel sequences and a further 7 that had previously been cloned only from mice.
Development of a micro-array to detect human and mouse microRNAs and characterization of expression in human organs.
A novel method to detect global processed miRNA expression levels in higher eukaryotes, including human, mouse and rats, by using a high-density oligonucleotide array is described and validated by subsequent confirmation of mir expression using northern-blot analysis.
Reduced expression of Dicer associated with poor prognosis in lung cancer patients
The results suggest the involvement of reduced Dicer expression in the development of lung cancers, thus warranting further investigations of the underlying mechanisms, which can be expected to enhance understanding of the molecular pathogenesis of this fatal cancer.
MicroRNA expression detected by oligonucleotide microarrays: system establishment and expression profiling in human tissues.
The established MIR-specific oligonucleotide microarray system that enables efficient analysis of the expression of the human MIRs identified so far is established, and novel data on MIR expression in thymus, testes, and placenta is presented.