MicroRNA expression in bovine preimplantation embryos.

@article{Berg2018MicroRNAEI,
  title={MicroRNA expression in bovine preimplantation embryos.},
  author={Debra K. Berg and Peter L. Pfeffer},
  journal={Reproduction, fertility, and development},
  year={2018},
  volume={30 3},
  pages={
          546-554
        }
}
We profiled 98 mature microRNAs (miRNAs) using a stem-loop reverse transcription polymerase chain reaction assay array based on human miRNAs. We demonstrated that one, but not two, base-pair changes in the miRNA recognition sequence at the 3' end only marginally affected copy number estimates. Absolute levels of miRNAs were measured in matured cattle oocytes, eight-cell embryos and normal and parthenogenetic blastocysts and Day-14 trophoblast. Most miRNA concentrations were below the expected… 
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Examining dynamic changes in relative abundance of sncRNAs during the maternal-to-embryonic transition (MET) in bovine embryos produced by scNT as compared to IVF by using RNA sequencing revealed largely similar profiles of snCRNAs for IVF and scNT embryos at the 2-cell, 8- cell, morula, and blastocyst stages of development.
The maternal-to-zygotic transition in bovine in vitro-fertilized embryos is associated with marked changes in small non-coding RNAs†
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A strong dynamic shift in the relative abundance of sncRNAs associated with the MZT in bovine oocytes and embryos is revealed, suggesting that these molecules may play important roles in the shift from maternal to zygotic control of gene expression.
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The role of miRNAs in mammalian gametogenesis and embryogenesis is highlighted and recent findings about miRNA-mediated post-transcriptional regulatory mechanisms occurring during early mammalian development are summarized.
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Comparative Analysis of Small Non-Coding RNA and Messenger RNA Expression in Somatic Cell Nuclear Transfer and In Vitro-Fertilized Bovine Embryos During Early Development Through the Maternal-to-Embryonic Transition
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A strong dynamic shift in relative abundance of differentially expressed sncRNAs is revealed, suggesting important roles for snc RNAs during the MET in bovine IVF embryos, and the dysregulation of sncRNA and transcriptome profiles in scNTbovine embryos compared to IVf embryos is assessed.
The Epigenetics of Gametes and Early Embryos and Potential Long-Range Consequences in Livestock Species—Filling in the Picture With Epigenomic Analyses
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The epigenetic analyses of germ cells and embryos in mammalian livestock species and the epigenetic determinants of gamete and embryo viability are reviewed and the effects of parental environmental exposures on the epigenetics of gametes and the early embryo are discussed.
Preimplantation development in ungulates: a "ménage à quatre" scenario.
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The body of work described here demonstrates the extent to which this dialogue enables the coordination of the development of the conceptus with respect to the establishment of embryonic and extra-embryonic tissues as well as in preparation for implantation.

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