MicroRNA cloning and sequencing in osteosarcoma cell lines: differential role of miR-93

@article{Montanini2011MicroRNACA,
  title={MicroRNA cloning and sequencing in osteosarcoma cell lines: differential role of miR-93},
  author={Luisa Montanini and Lisa Lasagna and Valeria Barili and S\oren Peter Jonstrup and Alba Murgia and L. Pazzaglia and Amalia Conti and Chiara Novello and J\orgen Kjems and Roberto Perris and Maria Serena Benassi},
  journal={Cellular Oncology},
  year={2011},
  volume={35},
  pages={29-41}
}
Studies show that abnormalities in non-coding genes can contribute to carcinogenesis; microRNA levels may modulate cancer growth and metastatic diffusion. MicroRNA libraries were built and sequenced from two osteosarcoma cell lines (MG-63 and 143B), which differ in proliferation and transmigration. By cloning and transfection, miR-93, expressed in both cell lines, was then investigated for its involvement in osteosarcoma progression. Six of the 19 miRNA identified were expressed in both cell… CONTINUE READING
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