MicroRNA 497 modulates interleukin 1 signalling via the MAPK/ERK pathway.

@article{Zheng2012MicroRNA4M,
  title={MicroRNA 497 modulates interleukin 1 signalling via the MAPK/ERK pathway.},
  author={Dongling Zheng and Anna Radziszewska and Patricia Woo},
  journal={FEBS letters},
  year={2012},
  volume={586 23},
  pages={4165-72}
}
The MAPK/ERK signalling pathway has been described to mediate IL-1 induction of target genes and is known to be regulated by microRNAs (miRNA). We describe a novel miRNA regulating the expression of the MEK1 gene and how it impacts IL-1 induced IL-6 transcription. miR-497 was predicted to target MEK1 3'UTR using bioinformatic tools. Transfection of miR-497 into HeLa cells inhibited MEK1 protein expression by 50%. In transient transfection experiments, the luciferase activity of a MEK1 3'UTR… CONTINUE READING