MicroRNA-185-5p mediates regulation of SREBP2 expression by hepatitis C virus core protein.

@article{Li2015MicroRNA1855pMR,
  title={MicroRNA-185-5p mediates regulation of SREBP2 expression by hepatitis C virus core protein.},
  author={Min Li and Qi Wang and Shun-ai Liu and Jin-qian Zhang and Wei Ju and Min Quan and Sheng-Hu Feng and Jin-Ling Dong and Ping Gao and Jun Cheng},
  journal={World journal of gastroenterology},
  year={2015},
  volume={21 15},
  pages={4517-25}
}
AIM To investigate the molecular mechanism for regulation of cholesterol metabolism by hepatitis C virus (HCV) core protein in HepG2 cells. METHODS HCV genotype 1b core protein was cloned and expressed in HepG2 cells. The cholesterol content was determined after transfection. The expression of sterol regulatory element binding protein 2 (SREBP2) and the rate-limiting enzyme in cholesterol synthesis (HMGCR) was measured by quantitative real-time PCR and immunoblotting after transfection. The… CONTINUE READING