Micro-scale extraction procedure for standardized screening of fungal metabolite production in cultures.

@article{Smedsgaard1997MicroscaleEP,
  title={Micro-scale extraction procedure for standardized screening of fungal metabolite production in cultures.},
  author={J\orn Smedsgaard},
  journal={Journal of chromatography. A},
  year={1997},
  volume={760 2},
  pages={264-70}
}
A simple and rapid standardized micro-scale extraction procedure has been developed to prepare extracts from fungal cultures for high-performance liquid chromatographic (HPLC) analysis. The method is based on ultrasonic extraction of three 6-mm plugs cut from a culture using 0.5 ml of solvent followed by a simple solvent change, filtration and injection. Approximately 5 min of work is involved in the extraction and work-up process and the extract can prepared for HPLC analysis within 60-70 min… CONTINUE READING
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