Micro high-performance liquid chromatographic separations of bile acids.

@article{Takeuchi1983MicroHL,
  title={Micro high-performance liquid chromatographic separations of bile acids.},
  author={Toyohide Takeuchi and Shingo Saito and Daiji Ishii},
  journal={Journal of chromatography},
  year={1983},
  volume={258},
  pages={125-34}
}
Separations of bile acids by micro high-performance liquid chromatography (micro-HPLC) using an immobilized 3 alpha-hydroxysteroid dehydrogenase post-column and a spectrofluorimeter are described. The sensitivity of detection is greatly increased by pre-mixing beta-nicotinamide adenine dinucleotide with the mobile phase, using a single pump. The detection limit is 0.04-0.7 ng, depending on the solute. Instruments suitable for the analysis of bile acids in micro-HPLC are discussed.