Micro- and nanoparticles of condensed DNA Formed in PCR with Taq polymerase and plasmid DNA as a template

@article{Danilevich2011MicroAN,
  title={Micro- and nanoparticles of condensed DNA Formed in PCR with Taq polymerase and plasmid DNA as a template},
  author={Vasily N. Danilevich and Ekaterina A. Vasilenko and Evgeniya V. Pechnikova and Olga S. Sokolova and Eugene V. Grishin},
  journal={Microbiology},
  year={2011},
  volume={80},
  pages={420-431}
}
Formation of micro- and nanoparticles of condensed DNA during PCR with microbial genomic DNA or plasmid DNA as templates was reported previously. Initially, the microparticles were formed using a thermostable KlenTaq polymerase, which is a deletion variant of Taq polymerase. The present work shows that Taq polymerase is also capable of efficient formation of micro- and nanoparticles of condensed DNA in PCR. Electron microscopy revealed a number of morphological types (more than four) of… CONTINUE READING

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