Micro-LED arrays: a tool for two-dimensional neuron stimulation

@article{Poher2008MicroLEDAA,
  title={Micro-LED arrays: a tool for two-dimensional neuron stimulation},
  author={Vincent Poher and Nir Grossman and G T Kennedy and Konstantin Nikolic and H. X. Zhang and Zheng Gong and Emmanuel M. Drakakis and Erdan Gu and Martin D. Dawson and Paul M.W. French and Patrick Degenaar and Mark A A Neil},
  journal={Journal of Physics D: Applied Physics},
  year={2008},
  volume={41},
  pages={094014}
}
Stimulating neuron cells with light is an exciting new technology that is revolutionizing the neurosciences. To date, due to the optical complexity that is involved, photostimulation has only been achieved at a single site using high power light sources. Here we present a GaN based micro-light emitting diode (LED) array that can open the way to multi-site photostimulation of neuron cells. The device is a two-dimensional array of micrometre size LED emitters. Each emitter has the required… 
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References

SHOWING 1-10 OF 35 REFERENCES
Flash photolysis using a light emitting diode: an efficient, compact, and affordable solution.
Rapid neurotransmitter uncaging in spatially defined patterns
TLDR
A new optical system for rapid uncaging in arbitrary patterns to emulate complex neural activity using TeO2 acousto-optical deflectors to steer an ultraviolet beam rapidly and can uncage at over 20,000 locations per second.
Efficient flip-chip InGaN micro-pixellated light-emitting diode arrays: promising candidates for micro-displays and colour conversion
Flip-chip InGaN micro-pixellated LED arrays with high pixel density and improved device performance are presented. The devices, with 64 × 64 elements, each of which have a 20 µm emission aperture on…
Light-activated ion channels for remote control of neuronal firing
TLDR
These synthetic photoisomerizable azobenzene-regulated K+ (SPARK) channels allow rapid, precise and reversible control over neuronal firing, with potential applications for dissecting neural circuits and controlling activity downstream from sites of neural damage or degeneration.
Microstripe-array InGaN light-emitting diodes with individually addressable elements
High-performance InGaN light-emitting diodes consisting of 120 side-by-side and individually addressable microstripe elements have been successfully fabricated. Each stripe in these devices is 24 mum…
Modeling and Engineering aspects of ChannelRhodopsin2 System for Neural Photostimulation
TLDR
A mathematical model for the photocycle of this protein, Channelrhodopsin-2, is developed, which gives results which are in good agreement with experimental measurements and engineering aspects of using this ChR2 system as a phototransduction mechanism are examined.
Kinetic evaluation of photosensitivity in genetically engineered neurons expressing green algae light-gated channels
Multimodal fast optical interrogation of neural circuitry
TLDR
An archaeal light-driven chloride pump from Natronomonas pharaonis is identified and developed for temporally precise optical inhibition of neural activity and forms a complete system for multimodal, high-speed, genetically targeted, all-optical interrogation of living neural circuits.
Long term recordings with microelectrode arrays: Studies of transcription-dependent neuronal plasticity and axonal regeneration
Confocal microscopic imaging of fast UV-laser photolysis of caged compounds
...
...