Mica functionalization for imaging of DNA and protein-DNA complexes with atomic force microscopy.

@article{Shlyakhtenko2013MicaFF,
  title={Mica functionalization for imaging of DNA and protein-DNA complexes with atomic force microscopy.},
  author={L. S. Shlyakhtenko and Alexander A. Gall and Yuri L Lyubchenko},
  journal={Methods in molecular biology},
  year={2013},
  volume={931},
  pages={295-312}
}
Surface preparation is a key step for reliable and reproducible imaging of DNA and protein-DNA complexes with atomic force microscopy (AFM). This article describes the approaches for chemical functionalization of the mica surface. One approach utilizes 3-aminopropyl-trietoxy silane (APTES), enabling one to obtain a smooth surface termed AP-mica. This surface binds nucleic acids and nucleoprotein complexes in a wide range of ionic strengths, in the absence of divalent cations and in a broad… CONTINUE READING

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