MiFish, a set of universal PCR primers for metabarcoding environmental DNA from fishes: detection of more than 230 subtropical marine species

@article{Miya2015MiFishAS,
  title={MiFish, a set of universal PCR primers for metabarcoding environmental DNA from fishes: detection of more than 230 subtropical marine species},
  author={M. Miya and Y. Sato and T. Fukunaga and T. Sado and J. Y. Poulsen and K. Sato and T. Minamoto and S. Yamamoto and H. Yamanaka and H. Araki and M. Kondoh and W. Iwasaki},
  journal={Royal Society Open Science},
  year={2015},
  volume={2}
}
  • M. Miya, Y. Sato, +9 authors W. Iwasaki
  • Published 2015
  • Medicine, Biology
  • Royal Society Open Science
  • We developed a set of universal PCR primers (MiFish-U/E) for metabarcoding environmental DNA (eDNA) from fishes. [...] Key Method Primers were designed using aligned whole mitochondrial genome (mitogenome) sequences from 880 species, supplemented by partial mitogenome sequences from 160 elasmobranchs (sharks and rays). The primers target a hypervariable region of the 12S rRNA gene (163–185 bp), which contains sufficient information to identify fishes to taxonomic family, genus and species except for some…Expand Abstract
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