MiFish, a set of universal PCR primers for metabarcoding environmental DNA from fishes: detection of more than 230 subtropical marine species

@inproceedings{Miya2015MiFishAS,
  title={MiFish, a set of universal PCR primers for metabarcoding environmental DNA from fishes: detection of more than 230 subtropical marine species},
  author={Masaki Miya and Yui Sato and Tsukasa Fukunaga and Tetsuya Sado and Jan Yde Poulsen and Keisuke Sato and Toshifumi Minamoto and Syuhei Yamamoto and Hiroki Yamanaka and Hitoshi Araki and Masashi Kondoh and Wataru Iwasaki},
  booktitle={Royal Society open science},
  year={2015}
}
We developed a set of universal PCR primers (MiFish-U/E) for metabarcoding environmental DNA (eDNA) from fishes. Primers were designed using aligned whole mitochondrial genome (mitogenome) sequences from 880 species, supplemented by partial mitogenome sequences from 160 elasmobranchs (sharks and rays). The primers target a hypervariable region of the 12S rRNA gene (163-185 bp), which contains sufficient information to identify fishes to taxonomic family, genus and species except for some… CONTINUE READING
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