Mg2+-induced compaction of single RNA molecules monitored by tethered particle microscopy.

Abstract

We have applied tethered particle microscopy (TPM) as a single molecule analysis tool to studies of the conformational dynamics of poly-uridine(U) messenger (m)RNA and 16S ribosomal (r)RNA molecules. Using stroboscopic total internal reflection illumination and rigorous selection criteria to distinguish from nonspecific tethering, we have tracked the… (More)

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@article{Lambert2006Mg2inducedCO, title={Mg2+-induced compaction of single RNA molecules monitored by tethered particle microscopy.}, author={Meredith Newby Lambert and Eva V{\"{o}cker and Seth Blumberg and Stefanie Redemann and Arivalagan Gajraj and Jens-Christian Meiners and Nils G. Walter}, journal={Biophysical journal}, year={2006}, volume={90 10}, pages={3672-85} }