Mg2+-induced compaction of single RNA molecules monitored by tethered particle microscopy.

@article{Lambert2006Mg2inducedCO,
  title={Mg2+-induced compaction of single RNA molecules monitored by tethered particle microscopy.},
  author={Meredith Newby Lambert and Eva V{\"o}cker and Seth Blumberg and Stefanie Redemann and Arivalagan Gajraj and J F Meiners and Nils G Walter},
  journal={Biophysical journal},
  year={2006},
  volume={90 10},
  pages={3672-85}
}
We have applied tethered particle microscopy (TPM) as a single molecule analysis tool to studies of the conformational dynamics of poly-uridine(U) messenger (m)RNA and 16S ribosomal (r)RNA molecules. Using stroboscopic total internal reflection illumination and rigorous selection criteria to distinguish from nonspecific tethering, we have tracked the nanometer-scale Brownian motion of RNA-tethered fluorescent microspheres in all three dimensions at pH 7.5, 22 degrees C, in 10 mM or 100 mM NaCl… CONTINUE READING