Metronidazole reduces the expression of cytochrome P450 enzymes in HepaRG cells and cryopreserved human hepatocytes

@article{Kudo2015MetronidazoleRT,
  title={Metronidazole reduces the expression of cytochrome P450 enzymes in HepaRG cells and cryopreserved human hepatocytes},
  author={Toshiyuki Kudo and Y. Endo and Rina Taguchi and Masami Yatsu and Kiyomi Ito},
  journal={Xenobiotica},
  year={2015},
  volume={45},
  pages={413 - 419}
}
Abstract 1. Blood levels of S-warfarin have been reported to be increased by concomitant administration of metronidazole (MTZ), an antiprotozoal imidazole derivative. 2. To elucidate the mechanism of this interaction and to identify other possible drug-drug interactions, we conducted an in vitro study with the human hepatoma HepaRG cells and cryopreserved human hepatocytes on the ability of MTZ to reduce the expression of cytochrome P450 (CYP) as well as nuclear receptors that regulate the… 
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Application of modified Michaelis – Menten equations for determination of enzyme inducing and inhibiting drugs

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  • Biology, Chemistry
    BMC pharmacology & toxicology
  • 2021
Pharmacokinetic-pharmacodynamic translation can be optimally achieved by incorporating, newly modified Michaelis-Menten equations into pharmacokinetic formulas for clinical efficacy and safety of the enzyme inducing and inhibiting therapeutic agents used in laboratory and clinical settings.

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The experimental results showed that the liver chimerism of the mice was adequate to support chronic HBV infection for 24 weeks and to evaluate antivirals, and overall, HepaRG-FRGS mice provide a novel human liver chimeric mouse model to study chronicHBV infection and evaluate anti-HBV drugs.

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Co-trimoxazole, metronidazoles, and itraconazole increase the risk of overanticoagulation in patients using acenocoumarol, and these combinations should be avoided if possible or otherwise acenOCou marol doses should be reduced and INR measured more frequently.

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