Methylumbelliferyl-N-acetylneuraminic acid sialidase in human liver.


Human liver sialidase was measured using methylumbelliferyl-N-acetylneuraminic acid as a substrate. The enzyme activity was linear for only 20 min and linearity was not improved by adding albumin, CaCl2, dithiothreitol, or Ep-459. The optimal pH was 4.5 and the apparent Km value, approximately 0.090 mM. Without substrate addition, the enzyme was unstable at… (More)


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@article{Tandt1984MethylumbelliferylNacetylneuraminicA, title={Methylumbelliferyl-N-acetylneuraminic acid sialidase in human liver.}, author={W R den Tandt and Simon S Scharp{\'e}}, journal={Biochemical medicine}, year={1984}, volume={31 3}, pages={287-93} }