Methyltransferases prefer monomer over core-trimmed nucleosomes as in vitro substrates.

  title={Methyltransferases prefer monomer over core-trimmed nucleosomes as in vitro substrates.},
  author={Yong Jiang and Jessica L Schneck and Maggie M Grimes and A Newman Taylor and Wangfang Hou and Sara H Thrall and Sharon M. Sweitzer},
  journal={Analytical biochemistry},
  volume={415 1},
Epigenetics is an area of increasing interest for drug discovery, driving the need for assays that use nucleosome substrates. Our studies showed that SUV39H1, a histone lysine methyltransferase, and Dnmt3b/Dnmt3L, a DNA methyltransferase, both exhibited approximately five times more activity on monomer nucleosomes than on DNA-core-trimmed nucleosomes in a scintillation proximity assay (SPA). The methyltransferases recognize and have a preference for nucleosomes with longer DNA strands. Our… CONTINUE READING

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