Methyltetrahydromethanopterin as an intermediate in methanogenesis from acetate in Methanosarcina barkeri

@article{Fischer2004MethyltetrahydromethanopterinAA,
  title={Methyltetrahydromethanopterin as an intermediate in methanogenesis from acetate in Methanosarcina barkeri},
  author={Reinhard Fischer and Rudolf Kurt Thauer},
  journal={Archives of Microbiology},
  year={2004},
  volume={151},
  pages={459-465}
}
Cell extracts (100,000×g) of acetate grown Methanosarcina barkeri (strain MS) catalyzed CH4 and CO2 formation from acetyl-CoA with specific activities of 50 nmol·min-1·mg protein-1. CH4 formation was found to be dependent on tetrahydromethanopterin (H4MPT) (apparent K M=4 μM), coenzyme M (H-S-CoM), and 7-mercaptoheptanoylthreonine phosphate (H-S-HTP=component B) rather than on methanofuran (MFR) and coenzyme F420 (F420). Methyl-H4MPT was identified as an intermediate. This compound accumulated… CONTINUE READING

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