Methylotrophic yeast Pichia pastoris as a host for production of ATP-diphosphohydrolase (apyrase) from potato tubers (Solanum tuberosum).

@article{Nourizad2003MethylotrophicYP,
  title={Methylotrophic yeast Pichia pastoris as a host for production of ATP-diphosphohydrolase (apyrase) from potato tubers (Solanum tuberosum).},
  author={Nader Nourizad and Maria Ehn and Baback Gharizadeh and Sophia Hober and P{\aa}l Nyr{\'e}n},
  journal={Protein expression and purification},
  year={2003},
  volume={27 2},
  pages={229-37}
}
ATP-diphosphohydrolase (apyrase) catalyzes the hydrolysis of phosphoanhydride bonds of nucleoside tri- and di-phosphates in the presence of divalent cations. This enzyme has broad substrate specificity for nucleotides, which makes it an ideal enzyme for different biotechnical applications, such as DNA sequencing and platelet-aggregation inhibition. The only commercially available apyrase is isolated from potato tubers. To avoid batch-to-batch variations in activity and quality, we decided to… CONTINUE READING